International Journal of the Humanities and Social Sciences

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PANTAO, an International Journal of the Humanities and Social Sciences, with ISSN 3028-0877, is an annual journal that serves as a scholarly platform dedicated to the exploration and dissemination of research in the disciplines of humanities and social sciences.

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Authors

Hugh Ariff M. Aserit
Bulacan State University
Malolos City, Bulacan, Philippines

Romer Cholo S. Cruz*
Bulacan State University
Malolos City, Bulacan, Philippines
Email: romercruz147@gmail.com

Denver Lyndon Aerol S. San Diego
Bulacan State University
Malolos City, Bulacan, Philippines

Allane Allan Paul N. Valenzuela
Bulacan State University
Malolos City, Bulacan, Philippines

Lech Walesa M. Navarra
Bulacan State University
Malolos City, Bulacan, Philippines

Abstract

In public environments, cleanliness and hygiene is significant aspect to avoid diseases, especially in facilities like restrooms and canteens. Although some may appear visually clean, it may still harbor bacterial presence. The BactoClean research assessed and evaluated bacterial contamination in high-traffic facilities within Bulacan State University. Data samples were gathered from frequently touched areas like door handles, flush handles, faucets, utensils, dining tables, food preparation area, respectively form restroom and canteen facility. Bacteria levels were measured as colony forming units and evaluated as CFU/cm². The samples were gathered and observed utilizing agar plates, and collected from both female and male in restrooms to avoid data biasing and alteration. A quantitative descriptive research design was applied and observational assessments were conducted, evaluating hygiene supply availability, and environmental practices for each facility. Results shown that College of Engineering showed the highest CFU count, while the College of Social Sciences and Psychology restroom recorded the lowest. Faucets and flush handles specifically present consistent higher CFU/cm² rating, recognizing them as bacterial reservoir. All the facility has shown bacterial presence, although it remained in low contamination spectrum. This study assists in continuous development of sanitation practices for maintaining a safe and healthy environment.

Keywords: Bacterial contamination, bacterial presence, sanitation practices, CFU/cm²

*Corresponding author
DOI: http://doi.org/10.69651/PIJHSS0501858

Recommended citation:
Aserit, H. A. M., Cruz, R. C. S., San Diego, D. L. A. S., Valenzuela, A. A. P. N., & Navarra, L. W. M. (2026). BactoClean: A conceptual assessment of hygiene conditions in university restrooms and canteens. Pantao (The International Journal of the Humanities and Social Sciences) 5 (1), 9666-9675. http://doi.org/10.69651/PIJHSS0501858

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References

Abudin, S., Martini, M., & Nurjazuli, N. (2023). Factors that trigger cockroach density: A literature review. Majalah Kesehatan Indonesia, 4(2), 71–76. https://doi.org/10.47679/makein.2023155

Al-Abady, A. J. (2022). A study of microbial contamination of toilets in the girls’ dormitory in Al-Qadisiya University. Journal of Wasit for Science and Medicine, 2(2). https://doi.org/10.31185/jwsm.59

Almatawah, Q., Al-Mutairi, S., & Varghese, J. (2024). Monitoring foodborne pathogens of food served at an institutional cafeteria. Applied Food Research, 4(2), 100497. https://doi.org/10.1016/j.afres.2024.100497

Alves, A., Viveiros, C., Lopes, J., Nogueira, A., Pires, B., Afonso, A. F., & Teixeira, C. (2021). Microbiological contamination in different food service units associated with food handling. Applied Sciences, 11(16), 7241. https://doi.org/10.3390/app11167241

Cappuccino, J. G., & Welsh, C. T. (2017). Microbiology: A laboratory manual (11th ed.). Pearson Education.

Chijioke, I., & Adaeze, C. N. (2024). Evaluation of Salmonella species, Escherichia coli, and Staphylococcus aureus associated with toilet door handles at girls and boys hostels in Federal Polytechnic of Oil and Gas Bonny. International Journal of Innovative Biochemistry & Microbiology Research, 12(2), 46–55. https://www.seahipublications.org/wp-content/uploads/2024/04/IJIBMR-J-4-2024.pdf

Dancer, S. J. (2004). How do we assess hospital cleaning? A proposal for microbiological standards for surface hygiene in hospitals. Journal of Hospital Infection, 56(1), 10–15. https://doi.org/10.1016/j.jhin.2003.09.017

Glowicz, J. B., Landon, E., Sickbert-Bennett, E. E., Aiello, A. E., deKay, K., Hoffmann, K. K., & Ellingson, K. D. (2023). SHEA/IDSA/APIC practice recommendation: Strategies to prevent healthcare-associated infections through hand hygiene: 2022 update. Infection Control & Hospital Epidemiology, 44(3), 355–376. https://doi.org/10.1017/ice.2022.304

Ibrahim, K., Tahsin, M., Rahman, A., Rahman, S. M., & Rahman, M. M. (2024). Surveillance of bacterial load and multidrug-resistant bacteria on surfaces of public restrooms. International Journal of Environmental Research and Public Health, 21(5), 574. https://doi.org/10.3390/ijerph21050574

International Organization for Standardization. (2019). ISO 18593: Microbiology of the food chain: Horizontal methods for surface sampling. ISO.

Jung, C., Samanoudy, G. E., & Alqassimi, N. (2024). Indoor air quality in educational institutions: A comparative study of VOCs and bacterial contaminants in Dubai schools. Frontiers in Built Environment, 10. https://doi.org/10.3389/fbuil.2024.1478681

Kabir, A., Roy, S., Begum, K., Kabir, A. H., & Miah, M. S. (2021). Factors influencing sanitation and hygiene practices among students in a public university in Bangladesh. PLOS ONE, 16(9), e0257663. https://doi.org/10.1371/journal.pone.0257663

Kramer, A., Schwebke, I., & Kampf, G. (2006). How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infectious Diseases, 6, Article 130. https://doi.org/10.1186/1471-2334-6-130

Nightingale, F. (1859). Notes on nursing: What it is, and what it is not. Harrison.

Pasteur, L. (1861). Mémoire sur les corpuscules organisés qui existent dans l’atmosphère. Comptes Rendus de l’Académie des Sciences.

Per, S., & Keleş Dinç, Y. (2024). Microbiological examination of washbasin, faucet heads, and toilet door handles of the students’ toilets at a state university. Celal Bayar University Journal of Science, 20(2), 19–27. https://doi.org/10.18466/cbayarfbe.1432698

Rahmiwati, A., Febry, F., Etrawati, F., et al. (2025). Food hygiene and sanitation of the university canteens in South Sumatra Province, Indonesia. Kesmas: National Public Health Journal, 20(2). https://doi.org/10.7454/kesmas.v20i2.2221

Triola, M. F. (2018). Elementary statistics (13th ed.). Pearson Education.

Tunio, A., Ahmed, J., Shaikh, M. Z., Channa, N., Hussain, S., & Baro, E. N. (2025). Impact of hand hygiene interventions on handwashing practices and microbial risk: A study in an orphanage-based school in Pakistan. American Journal of Infection Control, 53(1), 44–52. https://doi.org/10.1016/j.ajic.2024.09.008

Viegas, C., Pimenta, R., Dias, M., Gomes, B., Brito, M., Aranha Caetano, L., Carolino, E., & Gomes, A. Q. (2021). Microbiological contamination assessment in higher education institutes. Atmosphere, 12(8), 1079. https://doi.org/10.3390/atmos12081079

Wongsawan, K., et al. (2020). Existence of bacterial contamination on inanimate surfaces and equipment in sub-district health-promoting hospitals in Chiang Rai, Thailand. Journal of Current Science and Technology, 10(1), 55–63. https://ph04.tci-thaijo.org/index.php/JCST/article/view/320

World Health Organization. (2017). Guidelines on sanitation and health. World Health Organization.

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